MCB I Block 4 Notes

Download Here: MCB I – Block 4 Notes
1. MOLECULAR DIAGNOSTICS OF MUTATIONS

Sickle cell = caused by point mutation

Therefore you can use ASO to detect it,
but since this point mutation also alter restriction enzyme site (RFLP), you can also use Southern Blot, or PCR the fragment, then digest/run through gel.

Multiple […]

MCB I Block 3 Notes

Download Here: MCB I – Block 3 Notes
CELL MEMBRANE DISORDERS

Acanthocytosis/Spur Cell Anemia:

Too much alcohol or cholesterol → extra cholesterol accumulate in outer leaflet → increases surface area of outer bilayer → Less deformable → thorny → RBC destroyed by spleen → anemia
Destruction of liver → can’t […]